我有一篇英文的关于条件基因敲除打靶载体构建的专业文章，需要找个相关专业的研究生来帮我校对一下。文章我已经翻译出来了，由于我不是生物专业的，翻译的专业性可能不够。希望你能帮我改得专业一些。我翻译完后word中有10页，外加几个简单的图。我个人感觉不是很难，里面有很多操作步骤很容易翻译，因此修改也应该不难。
时间1-2天, 报酬200吧，我也是学生银两有限，希望哪位大侠帮我改一下。

以下是该文章摘要和我翻译，你觉得能够胜任的话就给我电话吧。（需要说明的是这篇文章我要做成PPT讲给别人听的，所以可不能闹笑话啊，哈哈～）
我的电话是15210264776
QQ:1206331131

Functional analysis of mammalian genes in vivo is
primarily achieved through analysing knockout
mice. Now that the sequencing of several mammalian
genomes has been completed, understanding
functions of all the genes represents the next major
challenge in the post-genome era. Generation of
knockout mutant mice has currently been achieved
by many research groups but only by making
individual knockouts, one by one. New technological
advances and the refinements of existing
technologies are critical for genome-wide targeted
mutagenesis in the mouse. We describe here new
recombineering reagents and protocols that enable
recombineering to be carried out in a 96-well
format. Consequently, we are able to construct
96 conditional knockout targeting vectors simultaneously.
Our new recombineering system makes it
a reality to generate large numbers of precisely
engineered DNA constructs for functional genomics
studies.

主要通过基因敲除的小鼠的手段来活体研究哺乳动物的基因功能。目前已有数条哺乳动物的基因已经完成测序，了解所有基因的功能是后基因时代的主要挑战。许多研究组已经成功的敲除了小鼠的基因，但是他们都是逐个的敲除。新技术的进步和现有技术的精炼对小鼠全基因组范围内定位突变至关重要。这里我们报道了能够在96孔板上进行重组试剂和步骤。结果显示我们能同时构建96个条件基因敲除的载体。我们新的重组系统使功能基因研究所需要的大规模精确设计DNA结构成为可能。
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