文章我已经翻译成中文了,我也是学生,但不是这个专业的,翻译得可能不准确,希望
找一个有相关背景的研究生帮我润色纠正一下。
要求:由于是要做成PPT讲的,所以文章中专业词汇或描述要翻译准确一点,忠于原
文,文章的遣词造句倒是其次。主要想让你帮改改专业的东西,对照一下原文来改,而
不是只挑里面的专业词汇“点对点”的翻译修改。
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下面是文章的出处:Nucleic Acids Research, 2011, Vol. 39, No. 17
下面列出原文摘要和我的翻译,供参考
原文题目:Efficient gene targeting mediated by a lentiviral vector-
associated meganuclease
原文摘要:
Gene targeting can be achieved with lentiviral vectors delivering donor
sequences along with a nuclease that creates a locus-specific double-strand
break (DSB). Therapeutic applications of this system would require an
appropriate control of the amount of endonuclease delivered to the target
cells, and potentially toxic sustained expression must be avoided. Here, we
show that the nuclease can be transferred into cells as a protein associated
with a lentiviral vector particle. I-SceI, a prototypic meganuclease from
yeast, was incorporated into the virions as a fusion with Vpr, an HIV
accessory protein. Integration-deficient lentiviral vectors containing the
donor sequences and the I-SceI fusion protein were tested in reporter cells
in which targeting events were scored by the repair of a puromycin
resistance gene. Molecular analysis of the targeted locus indicated a 2-fold
higher frequency of the expected recombination event when the nuclease was
delivered as a protein rather than encoded by a separate vector. In both
systems, a proportion of clones displayed multiple integrated copies of the
donor sequences, either as tandems at the targeted locus or at unrelated
loci. These integration patterns were dependent upon the mode of
meganuclease delivery, suggesting distinct recombination processes.
翻译题目: 一个与慢病毒载体有关的meganuclease(大范围核酸酶)介导的高效基因
打靶
摘要翻译: 慢病毒载体通过传递一个供体序列和一个能够产生基因组特异性断裂的核
酸酶来实现基因打靶(DSB)。临床上运用这种系统需要合适地控制传递到打靶细胞的
核酸内切酶的用量,避免潜在的毒性持续表达。这里我们介绍这种核酸酶能够作为一种
慢病毒颗粒有关的蛋白转移到细胞体内。一个来自酵母的原型的大范围核酸酶I-Scel通
过与一个HIV辅助蛋白Vpr融合而整合到病毒粒子上。整合缺陷型慢病毒载体(含有供体
序列和I-Scel融合蛋白)在报告细胞中筛查,只有那些含有嘌呤霉素抗性基因的才是正
确的打靶。分子手段分析打靶的基因座显示当核酸酶作为一个蛋白而不是被另一个分开
的载体编码情况下预期的重组频率要高2倍。在两个系统中,一部分的克隆显示出供体
序列的多拷贝克隆,或是在打靶基因座处或是在不相关的基因座串联起来。这些整合结
果取决于大范围核酸酶传递的模式,表明完全不同的重组过程。
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